control strain e coli atcc 25922  (ATCC)

Journal: Comparative Immunology Reports

Article Title: The synthetic peptide GATR-3 shows significant antibacterial and biofilm-inhibition activity against shellfish- and oyster-associated bacteria Vibrio vulnificus and Vibrio parahaemolyticus

doi: 10.1016/j.cirep.2025.200266

Figure Lengend Snippet: EC 50 results for Vibrio strains against GATR-3, LL-37-NH 2 , and Mastoparan-AF-NH 2 peptides. Vibrio vulnificus MO6 EC 50 results against A. GATR-3, B. LL-37-NH 2 , and C. Mastoparan-AF-NH 2 peptides. Vibrio vulnificus JY1701 EC 50 results against D. GATR-3, E. LL-37-NH 2 , and F. Mastoparan-AF-NH 2 peptides. Vibrio parahaemolyticus NY477 EC 50 results against G. GATR-3, H. LL-37-NH 2 , and I. Mastoparan-AF-NH 2 peptides. Vibrio parahaemolyticus SAK11 EC 50 results against J. GATR-3, K. LL-37-NH 2 , and L. Mastoparan-AF-NH 2 peptides. Escherichia coli EC 50 results against M. GATR-3, N. LL-37-NH 2 , and O. Mastoparan-AF-NH 2 peptides.

Article Snippet: Against the control strain E. coli ATCC 25922, GATR-3 was extremely potent, with an EC50 of 4.44 × 10−5 μM (1.27 × 10−4 μg/mL), confirming its broad-spectrum and potent efficacy.

Techniques:

Journal: Comparative Immunology Reports

Article Title: The synthetic peptide GATR-3 shows significant antibacterial and biofilm-inhibition activity against shellfish- and oyster-associated bacteria Vibrio vulnificus and Vibrio parahaemolyticus

doi: 10.1016/j.cirep.2025.200266

Figure Lengend Snippet: Biofilm Formation of Vibrio isolates and E. coli . Quantitative comparison of biofilm formation among Vibrio isolates and E. coli after 24 h incubation. Biofilm biomass was determined by crystal violet staining and measurement of OD₆₀₀. V. vulnificus MO6 and V. parahaemolyticus NY477 exhibited the highest biofilm-forming capacities.

Article Snippet: Against the control strain E. coli ATCC 25922, GATR-3 was extremely potent, with an EC50 of 4.44 × 10−5 μM (1.27 × 10−4 μg/mL), confirming its broad-spectrum and potent efficacy.

Techniques: Comparison, Incubation, Staining

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Assessment of PAS effects on planktonic K. quasipneumoniae ATCC 700603. A – E Bacterial growth curves under different treatment conditions (as indicated in the diagrams). The concentrations of CAZ as the fold change in the MIC (MIC CAZ = 32 mg/L) and phage titers are indicated in the legends. The data are presented as the means ± standard deviations (SDs, n = 3)

Article Snippet: Notably, the widely used quality-control strain ATCC 700603, originally labeled K. pneumoniae , has since been reclassified as K. quasipneumoniae subsp. similipneumoniae (hereafter referred to simply as K. quasipneumoniae ) [ ].

Techniques:

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Quantitative evaluation of treatment effects on K. quasipneumoniae ATCC 700603 biofilms following exposure to CAZ (0.25 × MIC), phage vB_KpUKJ_2 (10 8 PFU/mL), or their combination. A Surface area covered (SAC) by viable biofilm (in %) over time, as determined by the calcein fluorescence signal after staining with CAM. A total pixel area of 2048 × 2048 µm was analyzed from LSFM micrographs; B AUC representation of the kinetic data from ( A ) over a treatment period of 0–24 h, based on relative fluorescence units (RFU) × h; C Viable bacterial counts (CFU/mL) after biofilm treatments at three distinct time points. Statistical analysis was performed via two-way ANOVA followed by Tukey’s multiple comparisons test. D Phage particles (PFU/mL) after combined biofilm treatment at three time points. Statistical analysis was conducted via one-way ANOVA followed by Dunnett’s post hoc test. The data are presented as the means ± SDs (n = 3). Significance was assumed at p < 0.05 and is indicated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: Notably, the widely used quality-control strain ATCC 700603, originally labeled K. pneumoniae , has since been reclassified as K. quasipneumoniae subsp. similipneumoniae (hereafter referred to simply as K. quasipneumoniae ) [ ].

Techniques: Fluorescence, Staining

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Representative CLSM images showing the effects of phage vB_KpUKJ_2 (10 8 PFU/mL) on mature K. quasipneumoniae ATCC 700603 biofilms at 0, 4, and 24 h post-treatment. Biofilms were stained with Con-A (green) for α-polysaccharides or with CFW (magenta) for β-polysaccharides to visualize extracellular matrix integrity. A Untreated control, B phage-treated samples. To minimize photobleaching and laser-induced biofilm disruption, independent samples were used for each time point. Each image represents a representative Z-stack projection from three independent experiments. Scale bars: 20 μm

Article Snippet: Notably, the widely used quality-control strain ATCC 700603, originally labeled K. pneumoniae , has since been reclassified as K. quasipneumoniae subsp. similipneumoniae (hereafter referred to simply as K. quasipneumoniae ) [ ].

Techniques: Staining, Control, Disruption

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Characteristics of six phage-resistant mutants in comparison with the parental K. quasipneumoniae ATCC 700603 strain. A Differences in the AUCs based on growth kinetics. B Biofilm formation ability at 48 h quantified by crystal violet staining and absorbance measured at 570 nm; the data are presented as the means ± SDs (n = 3). Statistical analyses were performed via one-way ANOVA followed by Tukey’s multiple comparisons test. Significance was assumed at p < 0.05 and is indicated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0. 0001

Article Snippet: Notably, the widely used quality-control strain ATCC 700603, originally labeled K. pneumoniae , has since been reclassified as K. quasipneumoniae subsp. similipneumoniae (hereafter referred to simply as K. quasipneumoniae ) [ ].

Techniques: Comparison, Staining

Journal: Microbiology Spectrum

Article Title: Clinical characteristics and molecular evolution of ST11-KL64 carbapenem-resistant hypervirulent Klebsiella pneumoniae co-producing KPC-2 and NDM-1 from China

doi: 10.1128/spectrum.02911-25

Figure Lengend Snippet: Growth curves and stability of KPC-2-NDM-1-CRKPs. ( a ) Growth curves of six KPC-2-NDM-1-CRKPs, quality control standard strain ATCC BAA-1705 (KPC-2), and ATCC BAA-2146 (NDM-1). ( b ) Stability of bla KPC-2 and bla NDM-1 plasmids along 10-day serial passage.

Article Snippet: A total of eight K. pneumoniae strains were used, including six clinical isolates co-harboring bla KPC-2 and bla NDM-1 (KP1137, KP1225, KP1296, KP1316, KP1434, and KP1436), along with control strains ATCC BAA-1705 (producing only KPC-2) and ATCC BAA-2146 (producing only NDM-1).

Techniques: Control